diff --git a/httprunner/loader.py b/httprunner/loader.py
index 75d8e535..07761bcf 100644
--- a/httprunner/loader.py
+++ b/httprunner/loader.py
@@ -192,9 +192,9 @@ def load_dot_env_file():
     with io.open(path, 'r', encoding='utf-8') as fp:
         for line in fp:
             if "=" in line:
-                variable, value = line.split("=")
+                variable, value = line.split("=", maxsplit=1)
             elif ":" in line:
-                variable, value = line.split(":")
+                variable, value = line.split(":", maxsplit=1)
             else:
                 raise exceptions.FileFormatError(".env format error")
 
diff --git a/tests/data/test.env b/tests/data/test.env
index 09816d83..74d5d9ec 100644
--- a/tests/data/test.env
+++ b/tests/data/test.env
@@ -1,3 +1,4 @@
 UserName=test
 Password=654321
-PROJECT_KEY=AAABBBCCC
\ No newline at end of file
+PROJECT_KEY=AAABBBCCC
+content_type=application/json; charset=UTF-8
\ No newline at end of file
diff --git a/tests/test_loader.py b/tests/test_loader.py
index 18e67d86..03f8e4e0 100644
--- a/tests/test_loader.py
+++ b/tests/test_loader.py
@@ -148,6 +148,7 @@ class TestFileLoader(unittest.TestCase):
         env_variables_mapping = loader.load_dot_env_file()
         self.assertIn("PROJECT_KEY", env_variables_mapping)
         self.assertEqual(env_variables_mapping["UserName"], "test")
+        self.assertEqual(env_variables_mapping["content_type"], "application/json; charset=UTF-8")
         loader.dot_env_path = None
 
     def test_load_env_path_not_exist(self):